When using hexaphenoxycyclotriphosphazene, its samples need to be processed. In order to let you have some skills in this regard, the following will share the methods for you. Please understand them carefully.

　　1. Non reducing hexaphenoxy cyclotriphosphazene treatment

　　Physiological body fluid, serum, urea and other samples are generally boiled in 1% SDS boiling water for 3min without reducing agent. Therefore, the protein folding is not damaged and can not be used for the determination of molecular weight.

　　2. Reductive hexaphenoxy cyclotriphosphazene treatment with alkylation

　　The alkylation of iodoacetic amine can protect SH group well and firmly for a long time, and obtain a narrow band; In addition, iodoacetamide can trap excessive DTT and prevent the texture phenomenon during silver staining. 10ul of 20% iodoacetic acid amine in 100ul hexaphenoxy cyclotriphosphazene buffer and keep it at room temperature for 30min.

　　Among them, there is the sample of reduction treatment of hexaphenoxycyclotriphosphazene. In electrophoresis, the product is separated only according to the molecular weight. If you master these knowledge, the product will ensure its effect.